Coding

Part:BBa_K2785012:Experience

Designed by: Zemeng Wei   Group: iGEM18_Pittsburgh   (2018-10-09)


Applications of BBa_K2785012

We expressed the CDA protein as part of a fusion protein with dCas9 and ugi. The dCas9 functions a shuttle for the CDA to perform its deamination function at specific locations in the DNA.


T--Pittsburgh--dilution_actual_control.png |width="30%"|

T--Pittsburgh--Dilution_control.png

The results of our experiments illustrate that we get a linear increase in cytidine to thymine mutations at the correct base pair to be edited. These results match editing rate and function as previous papers have published. We performed 2-way ANOVA test to determine that there is a significant difference in editing between each 24-hour timepoint taken of our editing DNA.



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